Description
Description: KlenThermase DNA polymerase is an optimized version of DNA polymerase designed for cycle sequencing with dideoxynucleotides.
KlenThermase DNA polymerase is an optimised version of KlenTherm DNA polymerase designed for cycle sequencing with dideoxynucleotides. This enzyme is recommended both for manual DNA sequencing with 35S label and for automated fluorescent DNA sequencing. Mutations have been introduced into the KlenTherm DNA polymerase that confer on this enzyme enhanced properties for cycle sequencing of double-stranded PCR products. KlenThermase is similar to, yet distinct from, USB ThermoSequenase. We recommend to use KlenThermase with our thermostable Tth inorganic pyrophosphatase (1 unit of Tth inorganic pyrophosphatase added to 10 units of KlenThermase) for further improvement of uniformity of band intensities